Imagej fiji moving object two images4/30/2024 ![]() Images give everyone a chance to understand the problem.Notes on Quality Questions & Productive Participation I would love if some of you could point me in the right direction with this !! Now I would like to apply this mask to the original image as a selection (so it only selects the spheroids) and clear everything outside the selection.īackground ratiometric values are completely random noise and basically invariable through the experiment, so it's safe to just discard those pixels. Therefore, I decided to try the MorphoLibJ plugin, which does a fantastic job at segmenting my spheroids, from here I have created a binary mask. My final goal is to separate my cells (spheroids) from the background due to the nature of the image and very low signal-to-noise ratio of the autofluorphores, background substraction doesn't quite do the trick, because all pixels are between 0 and 1, so background can't be thresholded easily. I'm currently working with some ratiometric autofluorescence images (basically pixel intensity values are a result of some pixel math, which also makes all values fall between 0 and 1). ¹This subreddit is not affiliated with the creators of ImageJ or FIJI, but is simply a place to share ideas, papers, resources, and expertise - especially as relate to questions & answers posted here. Sign-up for one of the mailing lists: /Mailing_Lists It also hosts a forum for interacting with the developers.įIJI Is Just ImageJ - "a distribution of ImageJ (and ImageJ2) together with Java, Java3D and a lot of plugins organized into a coherent menu structure." is full of ImageJ development and analysis resources. Image analysis is interdisciplinary, so clearly explain field-specific terms or jargon. Clearly explain what you are trying to learn, not just the method used, to avoid the XY problem. Provide details: Be thorough in outlining the question(s) that you are trying to answer.People from the future may be stuck trying to answer the same question. Report spam or content that is hateful or off-topic. ![]() ![]() Upvote those who contribute to the discussion and provide freely of their time to assist you.Projects: Share a Link to your pet image analysis project.Research: Links to published (articles in scientific journals or in established repositories) that utilize ImageJ/FIJI for image analysis or are about image analysis.Discussions: Text posts, meant to ask about general issues relating to image analysis.Image analyst job posts are also welcome. Tips: Text or Link posts to share useful how-to tricks and discoveries on using ImageJ/FIJI.Questions which have been Solved will be marked as such. This could include algorithms, microscopy and scientific imaging, plug-ins, methods, and specific features of the software. Questions: Text posts asking about image analysis and ImageJ/FIJI.This is a an unofficial¹ forum to discuss image analysis, software features, to get help, to share ideas, and to share work done using ImageJ or FIJI. It's used worldwide, by a broad range of scientists. ImageJ is a freely available, open source image processing and analysis program using Java, on which FIJI is based.
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